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. 2020 Apr 6;11:1710. doi: 10.1038/s41467-020-15507-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Phylogeny of the 501 NCLDV MAGs presented in this study together with 121 reference genomes. The phylogeny was constructed from a concatenated alignment of 5 highly conserved marker genes that are present throughout the NCLDV families using the VT + F + I + G4 model in IQ-TREE. The tree is rooted at Poxviridae/Asfarviridae branch, consistent with previous studies¹¹. The inner strip is colored according to the phylogeny of the MAGs, while the outer strip is colored according to the habitat in which they were found. The bar chart represents genome size, which ranges from 100–2474 Kbp, and the dotted line denotes the 500 Kbp mark. Clades with >5 genomes are indicated with two letter abbreviations and clade numbers. MM: Mimiviridae, EP: Early Phycodnaviridae, LP: Late Phycodnaviridae, IR: Iridoviridae, MR: Marseilleviridae, PT: Pithoviridae. For the list of all the clades, see Dataset 1. b Average amino acid identity (AAI) heatmap of the MAGs and reference genomes, with rows and columns clustered according to the phylogeny.