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. 2020 Apr 6;11:1711. doi: 10.1038/s41467-020-15548-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Mammary tissue was dissociated and used to generate an organoid culture (ORG24) as well as a standard two-dimensional HMEC culture (HMEC24). Cells from the tissue was also directly fixed and frozen for future analysis. Cells from the cultures in conjunction with cells from the tissue were analyzed by CyTOF. a Heatmaps show single cells from the cultures or matched tissue as indicated, with color bar on left indicating different X-shift defined clusters. b Correlation between the protein expression profiles of HMEC or organoid cell and expression signatures derived from the major epithelial clusters in matched primary tissue. Box plots (center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range) show the maximum r value of each cell to the major epithelial clusters, stratified by sample. Statistical significance was assessed by two-sided Mann–Whitney test (***p < 2.2e−16). c Correlation analysis as in b, performed for the indicated ten organoid cultures as compared with a set of four nonmatching primary breast tissues.