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. 2020 Apr 6;10:5969. doi: 10.1038/s41598-020-62846-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HMEECs viability, apoptosis and reactive oxygen species production in presence of UP (200 µg/ml) or S. pneumoniae (MOI 10) or Co-treatment. (a) HMEECs viability upon UP or S. pneumoniae or Co-treatment. (b) Apoptosis detection using flow cytometry analysis of Annexin V/PI stained HMEECs treated with UP only or Streptococcus pneumoniae only or co-treatment (UP + Streptococcus pneumoniae. (c) Graphical representation of HMEECs percentage showed apoptosis. (d) ROS production detection in HMEEC treated with UP or Streptococcus pneumoniae or co-treatment. The error bars representing standard deviation from mean. The statistically significant were calculated by one-way ANOVA test and p-value less than 0.05 were considered significant.