Table 2.
The optimum formula for maximum pigment production by Natrialba sp. M6.
| Components | Basal medium | Optimized medium |
|---|---|---|
| Temperature (°C) | 37 | 45 |
| pH | 11 | 10.07 |
| Casaminoacids (%w/v) | 0.5 | 0.5 |
| NaCl (%w/v) | 20 | 25 |
| Na2CO3 (%w/v) | 0.9 | 0.9 |
| Agitation (rpm) | 200 | 200 |
| MgSO4.7H2O (%w/v) | 0.02 | 0.02 |
| KH2PO4 (%w/v) | 0.1 | 0.1 |
| Trace element solution* (ml %) | 0.1 | 0.1 |
| Old inoculum (days) | 7 | 11 |
| Final volume (ml) | 100 | 100 |
| Pigment (g/L) | 0.23 | 0.98226 |
*Trace element solution contains (g/L): ZnSO4.7H2O, 0.1; MnCl2.4H2O, 0.03; H3BO3, 0.3; CoCl2.6H2O, 0.2; CuCl2.2H2O, 0.01; NiCl2.6H2O, 0.02; and Na2MoO4.H2O, 0.03.