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. 2020 Apr 6;10:5956. doi: 10.1038/s41598-020-62673-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Graphical representation of DWV quasispecies composition change during transmission. Bubble-graph representation of the difference in frequency (f) of the consensus nucleotide (n) at each nucleotide position along the 10 kb DWV genome (nt) between the experimental sample (f(n_i)) and the original T0 inoculum (f(n_T0)), shown for each of the successive 24-hour transmission inocula (blue; T1–T8) and their corresponding 7-day symptomatic (light green) and asymptomatic (dark green) adult transmission progeny. The size of each bubble represents the extent of the change in the sub-consensus nucleotide frequency along the DWV genome, i.e. those quasispecies with the smallest bubbles and the least amplitude are most similar to the original T0 inoculum.