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. 2020 Feb 15;118(7):1679–1689. doi: 10.1016/j.bpj.2020.02.012

Figure 6.

Figure 6

The distal UbK48R in UBE2K-Ub2 interacts with the UBE2K UBA domain. (A) Expanded regions of 1H-15N TROSY spectra for 15N-labeled UBE2K alone (black) and in the UBE2K-UbG76C-UbK48R complex (magenta) are shown. Signals with significant shifts are labeled by residue and arrows. (B) A cartoon representation is given, showing residues whose signals underwent a significant chemical shift (>1 SD above the mean) upon addition of the distal UbK48R. (C) Expanded regions of 1H-15N HSQC spectra of 15N-labeled UbK48R in UbG76C-UbK48R (black) and UBE2K-UbG76C-UbK48R (red) are shown. Residues that shift in UbK48R upon formation of the conjugate are labeled. (D) A cartoon representation showing interacting residues on UbK48R is given. Signals that undergo significant chemical shift changes (>average + >1 SD) (pink) and residues whose signals experienced significant line broadening (purple) are indicated.