Figure 5.
(A and B) Shown are AFM data for low and high shear stress glycocalyx properties. Cells that were cultured under static conditions showed a significant decrease in thickness. There was no significant difference for the glycocalyx modulus for cells cultured. (C) Flow adhesion assay results are shown. Neutrophils were perfused over endothelial cells at physiological shear stress (0.25–0.5 dyn/cm2) for 5 min, and the number of adhering leukocytes was counted. Neutrophil capture depends on the shear rate at which they interact with the endothelium, which decreases with increasing shear stress. Cells cultured at low shear stress show significant increases in neutrophil adhesion with respect to cells cultured at 10 dyn/cm2. Error bars represent standard error. ANOVA was used to test for significance, p < 0.05. To see this figure in color, go online.