Figure 6.

AFM data for enzymatic treatment of HUVECS and pulmonary endothelial cell glycocalyx thickness. Cells that were enzymatically treated with either Heparinase III or hyaluronidase showed a significant decrease in thickness compared to untreated HUVEC. A combination of heparanase and hyaluronidase provided the largest measured effects for decreases in thickness. Pulmonary endothelial cells also had a significantly smaller glycocalyx than HUVECs. Error bars represent standard error. ANOVA was used to test for significance, p < 0.05. To see this figure in color, go online.