Estimates of motility coefficient and velocity from experimental and simulated data. (A) The histogram of the motility coefficients obtained for n = 403 cells, μexp = 1.61 ± 0.14 μm2 min−1 (mean ± standard error (SE)), is shown. (B) The histogram of the velocities obtained for the cells in (A), vexp = 0.51 ± 0.02 μm min−1 (mean ± SE), is shown. (C) The histogram of the motility coefficients obtained for n = 60 individual simulated trajectories with ψpol = 0.9, μsim = 7.27 ± 0.80 μm2 min−1 (mean ± SE) is shown. (D) The histogram of the velocities obtained for n = 60 individual simulated trajectories with ψpol = 0.9, vsim = 0.37 ± 0.04 μm min−1 (mean ± SE) is shown. Individual motility coefficients and velocities were obtained from fits to Eq. 2. All simulations were run with nmotor = 1000, nclutch = 750, and ψpol = 0.9, and all other parameter values reported in Table S1.