FIGURE 4.

Effect of GSH with Mg²⁺ or K⁺ on the growth of Zymomonas mobilis TIST R548. (A) Cells were grown in YPD medium with (open diamond) or without 0.5 mM GSH (control, closed diamond) at 39°C under a static condition. (B–D) Cells were grown in YPD medium containing 5 mM MgCl₂ (B), 30 mM KCl (C), or both 5 mM MgCl₂ and 30 mM KCl (D) with (open circle) or without 0.5 mM GSH (closed circle) at 39°C under a static condition. After 12 h, the first-stage culture (dotted lines) was inoculated into a fresh medium and subjected to subsequent (second stage) cultivation (solid lines) under the same medium condition. Additional and non-additional conditions of GSH are shown as open and closed circles, respectively. Values and error bars represent means and standard deviations, respectively, for triplicate cultures.