FIGURE 5.

Effect of EDTA with Mg²⁺ or K⁺ on the growth of Zymomonas mobilis TISTR 548. (A) Cells were subjected to two-step cultivation (dotted line, first; solid line, second) in YPD medium with 0.01 mM EDTA (open circle), 0.05 mM EDTA (open diamond) or without EDTA (closed circle) at 38°C under a static condition. (B,C) At the first stage of cultivation, cells were cultured in YPD medium containing 0.05 mM EDTA (open diamond) at 38°C, and after 12 h, at the second stage of cultivation (open circle), 5 mM MgCl₂ (B) or 30 mM KCl (C) was added to YPD medium containing 0.05 mM EDTA. Values and error bars represent means and standard deviations, respectively, for triplicate cultures.