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. 2020 Mar 31;11:405. doi: 10.3389/fimmu.2020.00405

Figure 1.

Figure 1

Non-phagocytic myeloid cells in peritoneal exudates contain monocytes and macrophages. Zymosan A (1 mg/mouse) was injected intraperitoneally to male mice. After 20, 44, or 68 h, these mice were injected I.P. with the phagocyte-specific dye PKH2-PCL green. Four hours later, the peritoneal cells were recovered and immunostained for F4/80 and CD11b. Dot plot analysis was performed for the expression of Ly6C (A, Y axis), CD11b (B), and PKH2-PCL acquisition (C), relative to F4/80 expression (X axis) by various exudate cells. Results are representatives from n = 8 mice for 24 h, six mice for 48 h, and seven mice for 72 h. (D–F) Peritoneal cells were recovered 66 h PPI and immunostained for CD11b, Ly6C, Ly6G, and F4/80 and analyzed by flow cytometry. Results are representative plots and means ± SEM (n = 12) showing CD11b+ gating (D), Ly6G vs. Ly6C (identifying monocytes and neutrophils, (E), and Ly6C vs. F4/80 (F).