Figure 3.

Ly6C^medF4/80^neg and Ly6C^hiF4/80^lo cells both convert to Ly6C^neg F4/80⁺ macrophages. Peritoneal exudates were recovered from WT mice 48 h PPI. (A) Monocytic cells were sorted into Ly6C^medF4/80^neg and Ly6C^hiF4/80^lo populations. (B–D) Sorted cells were labeled with CFSE and transferred to recipient mice with ongoing peritonitis at 48 h. At 72 h, peritoneal cells were recovered, immunostained for Ly6C and F4/80, and CFSE⁺ cells (B) were analyzed by flow cytometry (C, D). Results are stacked contour plot from six mice (C) and means ± SEM (n = 6). P < 0.001 (Student's t-test). (E,F) Peritoneal exudates were recovered from unchallenged mice or at 72 h PPI, immunostained for F4/80 and Tim4 and analyzed by flow cytometry. Results are stacked contour plots from six mice (E) and percentage means ± SEM of F4/80⁺ Tim4⁺ cells (F).***P < 0.001(Student's t-test).