Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 31;11:405. doi: 10.3389/fimmu.2020.00405

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Butenko, Satyanarayanan, Assi, Schif-Zuck, Barkan, Sher and Ariel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Satiated macrophages express the highest level of IFNβ of all resolution phase leukocytes. Peritoneal exudates were recovered from WT mice 66 h PPI, and the cells were immunostained for F4/80, fixed, permeabilized, and immunostained for IFNβ. (A) The gating strategy for eosinophils (green) and other immune cells. (B) Density plot analysis of F4/80 vs. PKH2 staining resulted in five distinct populations: F4/80⁻PKH2⁻ (cyan), F4/80⁻PKH2⁺ (yellow), F4/80⁺PKH2⁻ (blue), F4/80⁺PKH2^hi (red), and F4/80⁺PKH2^lo (purple). These populations were then analyzed for IFNβ expression, and results were presented as counts (C) and normalized to mode (D). MFI means ± SEM from six independent mice are shown (E). *P < 0.05, **P < 0.01, ***P < 0.001 (Student's t-test). Data for second antibody alone and eosinophils were previously reported in (17).