Figure 7.

Resolution phase macrophages resemble liver reparative Ly6C^lo macrophages and peritoneal monocyte-derived macrophages elicited by thioglycolate. Annotated genes were compared to the database of monocyte/macrophage populations from acute liver injury induced by overdose of N-acetyl-p-aminophenol (APAP) [Zigmond et al. (19)]. These subsets include inflammatory Ly6C^hi monocytes and their descendants, Ly6C^lo monocytes, as well as Kupffer cells from the steady state (KCSS) and recovered (KCR) phases. The 50 highest up- or downregulated genes in the liver Ly6C^lo differentiated macrophages were compared to PKH2^hi/phagocytic and PKH2^lo/^neg/non phagocytic macrophages (A). Differential distances of PKH2^hi and PKH2^lo/neg macrophages from liver macrophages and monocytes were visualized on a 3D PCA plot (B) and enumerated as PCA Euclidian distances (C). Alternatively, annotated genes were compared to the database of resident tissue macrophages and thioglycolate-elicited peritoneal monocyte/macrophage populations from the ImmGEN consortium (OpenSource mononuclear phagocyte project). The peritoneal resident populations were designated as RPM F4/80⁺ICAM2⁺ (F4/80⁺ICAM2⁺CD3⁻CD19⁻Ter119⁻) and RPM F4/80⁺ Tim4⁺/Tim4⁻ (B220⁻Ly6C⁻F480⁺CD11b⁺CD64⁺Tim4⁺/Tim4⁻). The peritoneal thioglycolate-elicited populations were designated as follows: monocytes 4 and 8 h Thio (CD45⁺CD11b⁺CD115⁺Ly-6C⁺ICAM2⁻CD226⁻), monocytes 24 h Thio (CD45⁺CD11b⁺CD115⁺Ly-6C⁺CD36^loICAM2⁻CD226⁻), and macrophages 24 and 72 h Thio (CD45⁺CD11b⁺CD115⁺Ly-6C^loCD36⁺ICAM2⁻CD226⁻). Differential distances of PKH2^hi and PKH2^lo/neg macrophages from peritoneal resident, and thioglycolate-elicited monocytes/macrophages were visualized on a 3D PCA plot (D) and enumerated as PCA Euclidian distances presented as group to group mean ± SEM (E). The 20 highest expressed genes (on CPM-TMM scale) from either PKH2^hi or PKH2^lo/neg macrophages were compared to monocytes 4 and 8 h Thio and macrophages 24 h Thio by RPKM values (F).