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. 2020 Mar 31;11:500. doi: 10.3389/fimmu.2020.00500

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Copyright © 2020 Sreekantapuram, Lehnert, Prauße, Berndt, Berens, Figge and Jacobsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune cell numbers in avian whole blood over the course of infection. Avian whole blood from the chicken lines WLA (left) and R11 (right) was infected ex-vivo with C. albicans, S. aureus, or E. coli for 240 min. Absolute numbers were determined for the different immune cell populations using flow cytometry and are depicted in percentage of the numbers at 0 min: Monocytes (A,F), heterophils (B,G), B cells (C,H), T cells (D,I), and thrombocytes (E,J). Data of six independent experiments using blood from different donors is presented as mean and SD. *indicates significant difference compared to 0 min (p < 0.05) with the color representing the respective condition: Blue: non-infected blood, purple: E. coli, green: S. aureus, red: C. albicans.