Table 5.

Enzyme levels for key reaction fluxes, calculated from enzyme turnover numbers 58 and rate maxima from the best-fit set.

Enzyme	Reaction	$k_{cat}$ (min−1), MOMENT	$V_{max}$ (mM/h), best-fit set	Enzyme Level (nM), calculated
Isocitrate dehydrogenase	R_icd	1700	37	356
Lactate dehydrogenase	R_ldh	52500	35	11
Aspartate transaminase	R_aspC	4900	39	130
Pyruvate kinase	R_pyk	8100	610	1250
Malic enzyme	R_maeA	8100	46	96
Malic enzyme	R_maeB	4000	66	274
Phosphofructokinase	R_pfk	5000	15600	51800
Malate dehydrogenase	R_mdh	43700	33	13
Succinate dehydrogenase	R_sdh	10000	4.9	8.2
Succinyl-coA synthetase	R_sucCD	1500	250	2690
Median				202