Table 6.

Reference values for transcription, translation, and mRNA degradation from literature. Transcription rate calculated from elongation rate, mRNA length, and promoter activity level. Translation rate calculated from elongation rate, protein length, and polysome amplification constant. mRNA degradation rate calculated from mRNA degradation time.

Description	Parameter	Value	Units	Reference
T7 RNA polymerase concentration	$R_T$	1.0	μM
Ribosome concentration	$R_X$	2	μM	Garamella et al. (2016)
Transcription saturation coefficient	$K_T$	100	nM	estimated
Translation saturation coefficient	$K_X$	45	μM	estimated
Transcription elongation rate	${\dot{v}}_T$	25	nt/s	Garamella et al. (2016)
CAT mRNA length	$l_G$	660	nt	Kigawa et al. (1995)
Promoter activity level	u	0.9		estimated
Transcription rate	$k_{cat}^T=\left(\frac{{\dot{v}}_T}{l_G}\right)u$	123	h−1	calculated
Translation elongation rate	${\dot{v}}_X$	1.5	aa/s	Garamella et al. (2016)
CAT protein length	$l_P$	219	aa	Kigawa et al. (1995)
Polysome amplification constant	$K_P$	10		estimated
Translation rate	$k_{cat}^X=\left(\frac{{\dot{v}}_X}{l_P}\right)K_P$	247	h−1	calculated
mRNA degradation time	$t_{1/2}$	8	min	BNID 106253
mRNA degradation rate	$k_{deg}=\frac{\text{ln}\left(2\right)}{t_{1/2}}$	5.2	h−1	calculated
ATP transcription coefficient	${\text{ATP}}_{\text{T}}$	176		calculated
CTP transcription coefficient	${\text{CTP}}_{\text{T}}$	144		calculated
GTP transcription coefficient	${\text{GTP}}_{\text{T}}$	151		calculated
UTP transcription coefficient	${\text{UTP}}_{\text{T}}$	189		calculated
ATP tRNA charging coefficient	${\text{ATP}}_{\text{X}}$	219		calculated
GTP translation coefficient	${\text{GTP}}_{\text{X}}$	438		calculated