Figure 11.

BTLA⁺ DCs in active TB patients were more readily polarized to Treg and Th2 in HCs but limited the Th17 and Th22 effector functions. BTLA⁺ DCs and BTLA⁻ DCs were sorted by flow cytometry, and host T cells were sorted by immune magnetic beads. BTLA⁺ DCs or BTLA⁻ DCs were co-cultured for 5 days with autologous T cells in the presence or absence of Mtb lysate antigen. On day 5, CD4⁺ T cell effector subsets were determined by intracellular cytokine staining. (A–E) show the comparison of frequencies of CD4⁺ T-cell effector subsets, Th1, Th2, Th17, Th22, and Treg, respectively, in APT (left) and HC (right) groups. (F) The graph data showing the frequencies of CD4⁺ T cell effector subsets, Th1, Th2, Th17, Th22, and Treg, respectively, in the co-cultures stimulated by Mtb lysate and autologous BTLA⁺ DCs from APT and HCs. The P-values are shown in each column (Student t-test). ^*P < 0.05, ^**P < 0.01.