Figure 5.

BTLA⁺ DCs in active TB showed a decreased MFI of HLA-DR, and reduced antigen uptake capacity. The MFI of the antigen presenting molecule HLA-DR in DC subsets was analyzed by flow cytometry. The antigen uptake capacity of DC subsets was evaluated by a FITC-dextran uptake test. (A) shows the MFI of HLA-DR expression between BTLA⁺ and BTLA⁻ tDCs and mDCs in HCs (n = 27), respectively. (B) shows the MFI of HLA-DR expression between BTLA⁺ and BTLA⁻ tDCs and mDCs in APT patients (n = 26). (C) shows the MFI of HLA-DR expression in BTLA⁺ tDCs, mDCs, and pDCs between HCs and APT patients. (D) Comparison of the MFI of HLA-DR expression in BTLA⁻ tDCs and mDCs between HCs and APT patients. (E,F) show the MFI of FITC-dextran uptake of BTLA⁺DCs and the counterpart BTLA⁻ DCs in HCs (E) and in APT patients (F). (G,H) show the MFI of FITC-dextran uptake in tDCs (G) and mDCs (H) from HCs (n = 10) and patients with APT (n = 10). The P-values are shown in each column (Student t-test). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.