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. 2020 Mar 31;11:518. doi: 10.3389/fimmu.2020.00518

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Copyright © 2020 Zhang, Lu, Wang, Liu, Chen, Shen, Luo, Xu, Peng, Luo, Huang, Wu, Zheng, Yi, Chen and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BTLA⁺ DCs in active TB showed an increased expression of the co-stimulatory molecule CD86. The expression of the co-stimulatory molecule CD86 in DCs was determined by flow cytometry (n = 8). The gating strategy is shown in Figure S1. (A,B) show the positive rate (%) (A) and MFI (B) of CD86 expression between BTLA⁺ DCs and the counterpart BTLA⁻ DCs in HCs. (C,D) show the positive rate (%) (C) and MFI (D) of CD86 expression between BTLA⁺ DCs and BTLA⁻ DCs in APT. (E,F) show the mean frequency (%) (E) and MFI (H) of CD86 expression in BTLA⁺ tDCs/mDCs/pDCs between HCs and APT patients. (G,H) show the mean frequency (%) (G) and MFI (H) of CD86 expression in BTLA⁻ tDCs and mDCs between HCs and APT patients. The P-values are shown in each column (Student t-test). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.