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. 2020 Mar 31;11:250. doi: 10.3389/fgene.2020.00250

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Copyright © 2020 Ma, Lei, Ding, Luo, Xie and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of NEAT1 suppresses tumor growth in vivo. Panels (A,B) BALB/c mice were challenged with 10⁶ M109 cells subcutaneously, and tumor volume was assessed over time. (C) Tumor weight was recorded. (D) Representative plots of CD3 staining. Numbers indicated frequency of CD3⁺ T cells. (E) Frequency of tumor CD3⁺ T cells was analyzed by flow cytometry. (F) Representative plots of CD8 staining. Numbers indicate frequency of CD8⁺ T cells. (G) Frequency of tumor CD8⁺ T cells was analyzed by flow cytometry. (H) Representative image of CD8 staining by IHC. (I) IHC staining score of intratumor CD8⁺ T cells. ***p < 0.001. Data are expressed as the mean ± SD.