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. 2020 Mar 31;11:530. doi: 10.3389/fimmu.2020.00530

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Copyright © 2020 Fromell, Adler, Åman, Manivel, Huang, Dührkop, Sandholm, Ekdahl and Nilsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Measurement of C3(x) formation at different pH using the C3(x) ELISA. (B) Virtual blot view of Wes immunoassay analysis of Bb generation after addition of FD to C3 at different pH. (C) The corresponding virtual blots for Bb generation after addition of FD to C3 incubated at different pH, followed by re-neutralization back to neutral pH (7.4).