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The molecular weight, and final concentration of complement proteins used in Wes immunoassay for analysis of C3 convertase formation.
| Protein | Molecular weight (kDa) | Concentration for incubation at 37°C (μg/mL). Diluted in VB++ | Final concentration in Wes Diluted 1:5 in 0.1 x Wes Sample Buffer (μg/mL) |
|---|---|---|---|
| Native C3 | 185 | 70 | 14 |
| C3b | 176 | 70 | 14 |
| C3 methylamine | 185 | 70 | 14 |
| C3 KSCN | 185 | 70 | 14 |
| Factor B | 93 | 100 | 20 |
| Factor D | 24 | 0.5 | 0.1 |
