FIGURE 4.
The optimum temperature and pH of the purified enzyme. (A) Optimum Temperature: the enzyme activity was measured at various temperatures (20–70°C) in 1 M NH4Cl-NH3⋅H2O buffer (pH 8.5). (B) Optimum pH: the enzyme activity was assayed at various pH (7.0–11.0) in NH4Cl-NH3⋅H2O buffer. Relative activity was expressed as a percentage of maximum activity under the experimental conditions.