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Effect of buffer pH on the reductive amination of TMP. Reaction conditions: TMP (0.3 M), ammonium formate (0.8 M), NAD+(0.25 g L–1) and cell-free extracts of E. coli cells (10 g L–1 EsiLeuDH, 20 g L–1 FDH) in PBS buffer (0.1 M), 30°C, 180 rpm, 12 h.
