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Effect of substrate feeding strategy on the reductive amination of TMP. Reaction conditions: four additions of TMP solution (1.5, 2, 4, 2.5 mL four times at 0, 6, 12, and 24 h, final concentration 0.8 M), ammonium formate (0.8 M), NAD+ (0.25 g L–1) and cell-free extracts of E. coli cells (10 g L–1 EsiLeuDH, 20 g L–1 FDH) in PBS buffer (0.1 M).
