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. 2020 Mar 31;8:186. doi: 10.3389/fbioe.2020.00186

TABLE 4.

Steady-state kinetic constants of EsiLeuDH.

Substrate Km Vmax kcat kcat/Km
(mM) (μmol⋅min–1⋅mg–1) (s–1) (L⋅mmol–1⋅s–1)
NADH 0.624 114.16 76.86 123.17
TMP 5.96 67.48 45.43 7.62
NAD 1.46 12.49 8.41 5.76
L-Leucine 0.88 6.4 4.31 4.90

EsiLeuDH, leucine dehydrogenase gene from Exiguobacterium sibiricum; TMP, trimethylpyruvic acid.