TABLE 4.
Substrate | Km | Vmax | kcat | kcat/Km |
(mM) | (μmol⋅min–1⋅mg–1) | (s–1) | (L⋅mmol–1⋅s–1) | |
NADH | 0.624 | 114.16 | 76.86 | 123.17 |
TMP | 5.96 | 67.48 | 45.43 | 7.62 |
NAD | 1.46 | 12.49 | 8.41 | 5.76 |
L-Leucine | 0.88 | 6.4 | 4.31 | 4.90 |
EsiLeuDH, leucine dehydrogenase gene from Exiguobacterium sibiricum; TMP, trimethylpyruvic acid.