Fig. 5.

FGF19-M52 does not affect colonic expression of genes involved in local pro-inflammatory immune response and epithelial barrier homeostasis In FXR^null mice. (a) qRT-PCR of proinflammatory genes IL-1β, IL-6 and TNFα. (b) Intestinal immunohistochemical staining of the inflammatory marker F4/80 (magnification 10X). Quantification of the signal assessed with Aperio Image Scope Software. qRT-PCR of (c) tight junctions’ genes Claudins 2, 3, 12 and Occludin and (d) intestinal differentiation markers Cdx2 and Klf4. Ct values are indicated. All values represent means±SEM. Statistical significance comparing AAV-FGF19-M52 versus control AAV-GFP assessed by Mann-Whitney's U test (*p < 0.05).