Fig. 4.

HIV-specific cTfh are predominantly Th1-polarized. (a) Example plot showing CXCR3/CCR6 expression on HIV-specific cTfh. (b) Comparison of Ag-specific cTfh subsets based on CXCR3/CCR6 coexpression. (c) Example plots showing expression of IFNγ in HIV- and CMV-specific cTfh. (d) Frequency of Th1-cytokine⁺ HIV- vs CMV-specific cTfh. (e) Frequency of HIV Gag-specific cTfh subsets expressing IFNγ, CXCL13 or IL-21. (f) Co-expression analysis of Ag-specific cTfh cells based on CXCL13, IFNγ and IL-21 expression. n = 27 (HIV), n = 23 (CMV) or n = 6 (HBV) for (b); n = 12 for (d), n = 9 for (e,f). P-values shown in figure were calculated by Kruskal–Wallis test with Dunn's post test (b), Wilcoxon test (d, f) or Friedman test with Dunn's post test (e).