Fig. 6.

HIV-specific cTfh responses correlate with p24- and gp120-specific antibody levels in ART-treated donors. Plasma samples from HIV⁺ donors were analyzed for HIV-specific Abs by ELISA (p24-Abs) or flow cytometry (gp120-Abs). (a) Longitudinal analysis of p24-specific Ab levels using matched plasma samples obtained during chronic untreated infection or during ART. (b) Level of p24- or gp120-specific Abs in ART-treated vs uninfected control (UC) donors. Dotted line represents limit of detection. (c) Longitudinal analysis of gp120-specific Abs using matched plasma samples from 2 different time points of long-term ART-treated subjects. (d) Correlation between p24- and gp120-specific Ab plasma levels. (e-g) Correlation between total HIV-specific CD4⁺ T cells (e), HIV-specific non-cTfh (f) or HIV-specific cTfh (g) and levels of p24- or gp120-specific Abs, respectively. n = 7 for (a); p24-Abs: n = 4 UC and n = 27 ART, gp120-Abs: n = 12 UC and n = 18 ART for (b); n = 5 for (c); n = 18 for (d); p24-Abs: n = 27, gp120-Abs: n = 18 for (e-g). Gray-bordered symbols represent values below limit of detection. P values were calculated by Wilcoxon test (a) or Spearman correlation (d-g).