Fig. 7.

Th1-like phenotype and function of HIV-specific cTfh correlates with size of the translation-competent reservoir. (a) Example plot showing CD4⁺ T cells from one representative ART-treated donor expressing HIV gag mRNA and HIV Gag protein after resting or stimulation with PMA/Ionomycin. (b) Frequency of HIV^RNA+/Gag+ CD4⁺ T cells in uninfected control (UC) or ART-treated individuals in resting CD4⁺ or after PMA/Ionomycin stimulation. Gray-bordered symbols are below limit of detection (dotted line). Lines represent median values. (c) Heatmap showing associations between phenotype and function of HIV-specific cTfh (left graph) or HIV-specific non-cTfh (right graph) with total HIV DNA, integrated HIV DNA and translation-competent reservoir. Color represents R value for each association calculated and *p < 0.05 indicates significant correlations (Spearman correlation). n = 3 UC (uninfected control donors), n = 11 ART for (b); n = 23 ART for correlations with total or integrated HIV DNA, n = 11 for correlations with translation-competent reservoir (c). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)