Table 2.
Cell viability of RD and L20B cells after 48-hour treatment with SAHA extracts and doxorubicin.
| SAHA extract | Concentration (μg/mL) | Cell viability (%) | |
|---|---|---|---|
| RD cells | L20B cells | ||
| Dichloromethane | 200 | 39.5 ± 5.1a | 64.5 ± 4.1∗ |
| Dichloromethane | 100 | 45.4 ± 9.7a | 71.4 ± 6.0∗ |
| Dichloromethane | 50 | 47.1 ± 5.7a | 72.0 ± 8.6∗ |
| Dichloromethane | 25 | 63.1 ± 4.6 | 78.2 ± 5.8∗ |
| Dichloromethane | 12.5 | 77.0 ± 7.3 | 85.6 ± 2.1 |
| Acetone | 200 | 43.4 ± 4.9a | 68.4 ± 7.2∗ |
| Acetone | 100 | 61.6 ± 3.5 | 72.3 ± 9.2∗ |
| Acetone | 50 | 73.3 ± 7.3 | 74.4 ± 12.1 |
| Acetone | 25 | 79.2 ± 5.6 | 74.3 ± 10.2 |
| Acetone | 12.5 | 84.3 ± 5.5 | 92.9 ± 5.7 |
| Methanol | 200 | 40.0 ± 6.2a | 79.3 ± 4.4∗∗∗ |
| Methanol | 100 | 48.3 ± 5.6a | 80.6 ± 8.9∗ |
| Methanol | 50 | 64.1 ± 3.5 | 86.0 ± 5.0∗ |
| Methanol | 25 | 63.8 ± 6.3 | 86.2 ± 3.0∗ |
| Methanol | 12.5 | 76.6 ± 10.1 | 89.7 ± 4.8 |
| Aqueous | 200 | 41.4 ± 3.0a | 62.5 ± 1.8∗ |
| Aqueous | 100 | 50.7 ± 1.6a | 63.2 ± 3.8 |
| Aqueous | 50 | 58.9 ± 5.8a | 66.9 ± 4.5 |
| Aqueous | 25 | 74.3 ± 4.0 | 70.3 ± 2.7 |
| Aqueous | 12.5 | 83.6 ± 1.3 | 79.4 ± 8.6 |
| Doxorubicin | 8 | 30.5 ± 2.4a | 59.2 ± 7.5b∗ |
| Doxorubicin | 4 | 34.2 ± 4.1a | 71.0 ± 9.4∗∗∗ |
| Doxorubicin | 2 | 40.1 ± 5.4a | 73.9 ± 6.1∗∗∗ |
| Doxorubicin | 1 | 44.4 ± 6.8a | 77.5 ± 10.0∗∗∗ |
| Doxorubicin | 0.5 | 57.2 ± 11.1a | 81.9 ± 5.3∗ |
The number of live negative control cells (0 μg/mL) at 48 h was used as 100% viability, so all cells were expressed as a percentage of them. Results are expressed as mean ± SD (n = 3). a and b represents significant difference (p < 0.001), compared to untreated RD and L20B cells, respectively. ∗ indicates p < 0.05 and ∗∗∗ indicates p < 0.0001 when comparing RD cells to L20B cells.