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. 2020 Mar 31;11:367. doi: 10.3389/fpls.2020.00367

FIGURE 3.

FIGURE 3

Over-expression of DEAR4 accelerates leaf senescence. (A) The phenotypes of DEAR4 over-expression lines. (B) Detached leaf phenotypes of Col-0, DEAR4-OE-3, and DEAR4-OE-5 described in (A). (C) Trypan blue staining of the sixth leaf of 6-week-old Col-0 and DEAR4 over-expression lines. (D) Total chlorophyll content of 1th–12th leaf from 6-week-old Col-0 and DEAR4 over-expression lines. (E) Leakage rate of Col-0 and DEAR4 over-expression lines. (F) Inducible over-expression of DEAR4 causes precocious senescence. (G) Chlorophyll content of different genotype plants that were treated with EST. (H) Trypan blue staining of different plants as indicated. The bars are standard deviations (SD) of three biological replicates. The one and double asterisks indicate significant difference to control at the levels of 0.01 < P < 0.05 and P < 0.01 using student’s t-test, respectively.