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. 2020 Mar 31;11:367. doi: 10.3389/fpls.2020.00367

FIGURE 8.

FIGURE 8

Transcriptional repression of DEAR4 on COR and RD genes. (A) The expression of COR15a, COR15b, RD29a, and RD29b in Col-0 and DEAR4 over-expression lines. (B) Dual luciferase (LUC) constructs used in this study. Dual LUC constructs contain the double reporter and effector plasmids. In the double reporter construct, Renilla LUC expression was used to normalize the firefly luciferase activities. The effector construct in this study contains DEAR4 driven by the CaMV35S promoter. (C) DEAR4 directly represses the expression of COR15 and RD29. The firefly LUC gene was driven by the COR15 and RD29 promoters, ratio of firefly and RenLuc indicates the relative activities of promoters. The bars are standard deviations (SD) of three biological replicates. The one and double asterisk indicate significant difference to control at the levels of 0.01 < P < 0.05 and P < 0.01 using student’s t-test, respectively.