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. Author manuscript; available in PMC: 2020 Sep 12.
Published in final edited form as: Nat Biomed Eng. 2020 Mar 12;4(3):272–285. doi: 10.1038/s41551-020-0526-9

Fig. 5 |. Rapid PARPi-FL staining on fresh biospecimens.

Fig. 5 |

a, Workflow of rapid PARPi-FL staining of fresh human biospecimens. Biospecimens were split and separately processed for fresh tissue staining as well as histopathology. b, Examples of PARPi-FL staining of a tumour and margin sample and corresponding histopathology of the same sample. We aimed to scan the entire fresh tissue in a high-resolution tile scan. Lower and higher magnification images showcase PARPi-FL staining and corresponding PARP1 expression in the specimens. A total of 22 tissues (n=12 tumours and n=10 benign tissues adjacent to tumour from 13 individual patients) were stained and analysed. The full data set is provided in supplementary Materials PDF files 1 and 2. c, In a blinded study, readers (n=27) scored 30 cases (n=12 tumours, n=10 margins, n=8 duplicates (4 tumours, 4 margins)) as tumour or margins (see fig. S9 for study design). Pairs of sensitivity and specificity for each reader are represented in the graph.