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. 2020 Mar 31;11:299. doi: 10.3389/fgene.2020.00299

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Copyright © 2020 Zhu, Liu, Xu, Liu, Xie, Sham, Sha and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Germline specific dgcr8 deletion has no functional influence on gonad development. (A) A basic schematic diagram of generating dgcr8 germline conditional knockout zebrafish. (B) Real-time analysis of the expression of dgcr8 mRNA and primary miRNAs in ovary of dgcr8 kop-cKO fish. (C) Real-time PCR analysis of the expression of mature miRNAs in ovary of dgcr8 kop-cKO fish. (D) Expression of germline markers in the ovary of dgcr8 kop-cKO. (E) Histology of ovaries in the wild-type and dgcr8 kop-cKO female zebrafish. *p < 0.05, **p < 0.01, and ****p < 0.0001.