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. 2020 Mar 31;11:299. doi: 10.3389/fgene.2020.00299

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Copyright © 2020 Zhu, Liu, Xu, Liu, Xie, Sham, Sha and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maternal mutants exhibit reduced erythropoiesis and could be rescued in Mdgcr8, but not in MZdgcr8. Whole embryo O-dianisidine staining assay was used for assessing functional hemoglobin in mature primitive erythrocytes in wild-type, Mdgcr8, MZdgcr8, and miR-430 rescued groups at 50 (A) and 74 hpf (B). Hemoglobinized cells accumulate in the yolk sac in wild-type but mainly accumulate in the caudal vein (red arrows) in MZdgcr8 and MZdgcr8 mutants. 10–15 embryos per groups. Scale bar: 200 μm. (C) The touch response was categorized as strong, weak, and no response based on the analysis of the video frames. (D) At 60 hpf, the rescue rate was determined in wild-type and maternal mutant embryos. (E) At 48 hpf, the hatching rate was detected in wild-type and maternal mutant embryos. ****p < 0.0001.