Figure 5.

Knockdown of GRP78 promotes BC cell sensitivity to 5-FU in nude mice. A. Body weight of nude mice. B. Tumor volume in nude mice. C. The representative tumors formed in nude mice. D. The tumor weight in nude mice. E. The expression of GRP78, OCT4, AKT, PERK, IRE1 and ATF6 in tumors formed in nude mice measured by Western blot analysis. F. The expression of MIAT in tumors formed in nude mice determined by RT-qPCR assay. *P<0.05 vs. nude mice injected with MCF-7/5-FU cells stably transfected with si-NC and PBS. Measurement data were expressed as mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post-hoc test. Statistical analysis in relation to time-based measurements within each group was realized using repeated measures ANOVA, followed by a Bonferroni’s post-hoc test for multiple comparisons.