Figure 7. RSKi combinatorial treatment induces apoptosis in 3D co‐culture and in vivo .

• A
  Confocal micrographs show cleaved caspase‐3 (clCasp3, green), mRFP (orange), and phalloidin (F‐actin, red; only shown for CAF) in 3D OVCAR8‐RFP and OCKI_p22 CAF mono‐ and co‐cultures treated without or with 20 µM cisplatin or 25 µM LJH685 alone or in combination for 20 h. Scale bars: 100 μm.
• B
  Quantitative assessment of clCasp3⁺ cells in the 3D cultures. N = 4.
• C
  Confocal micrographs show cytokeratin 7 (CK7, green) and phalloidin (F‐actin, red) in 3D patient OCKI_p13 mono‐ and co‐culture with OCKI_p22 CAF treated without or with 20 µM cisplatin or 25 µM LJH685 alone or in combination for 20 h. Scale bars: 50 μm.
• D
  Quantitative assessment of clCasp3⁺ OCKI_p13 cells in 3D co‐cultures of patient‐derived cells. N = 4.
• E–H
  Immunohistochemistry images and quantifications of TUNEL (E, F) and Ki67 (G, H) in OVCAR4 xenografts in mock (N = 4)‐, carboplatin (N = 4)‐, and carboplatin + BI‐D1870 (N = 5)‐treated mice. Scale bars: 100 μm.

Data information: In (B and D, F, and H), data are presented as mean (SD). *P < 0.05, **P < 0.01. Exact P‐values are provided in Appendix Table S10, Student's t‐test (B, D) and Mann–Whitney U‐test (F, H).