Figure 5. A151 attenuates neutrophil infiltration and production of pro‐inflammatory factors in microglia after tMCAO.

• A
  Gating strategy of brain‐infiltrating immune cells including CD4⁺ T cells (CD3⁺ CD4⁺), CD8⁺ T cells (CD3⁺CD8⁺), B cells (CD3⁻CD19⁺), NK cells (CD3⁻NK1.1⁺), monocyte/macrophages (CD11b⁺CD45^highF4/80⁺), neutrophils (CD11b⁺CD45^highLy6G⁺), and microglia (CD11b⁺CD45^int) and their expression of IL (interleukin)‐6, TNF‐α (tumor necrosis factor‐α), TGF‐β (transforming growth factor‐β), and IL‐4. FMO, fluorescence minus one.
• B
  Quantitative analysis of the counts of brain‐infiltrating leukocytes in the brains of MCAO mice receiving indicated treatment. n = 6 mice per group. **P < 0.01, two‐tailed unpaired Student's t‐test.
• C
  Bar graph shows counts of leukocyte subtype in the spleens of MCAO mice receiving indicated treatment. n = 6 mice per group. **P < 0.01, two‐tailed unpaired Student's t‐test.
• D
  Quantitative analysis of the microglia in the brains of MCAO mice receiving indicated treatment. n = 6 mice per group. *P < 0.05, two‐tailed unpaired Student's t‐test.
• E
  Bar graph shows percentages of microglia expressing IL‐6, TNF‐α, TGF‐β, and IL‐10 in the brains of MCAO mice receiving indicated treatment. n = 6 mice per group. *P < 0.05, **P < 0.01, two‐tailed unpaired Student's t‐test.
• F
  Representative images of immunofluorescent staining for ly6G in peri‐infarct areas of MCAO mice receiving indicated treatment. Scale bar, 50 μm.
• G
  Bar graph shows the number of ly6G⁺ neutrophils. n = 6 mice per group. **P < 0.01, two‐tailed unpaired Student's t‐test.
• H
  Representative images of immunofluorescent staining for Iba‐1 in peri‐infarct areas of MCAO mice receiving indicated treatment. Scale bar, 50 μm.
• I
  Bar graph shows the number of Iba‐1⁺ microglia. n = 6 mice per group. **P < 0.01, two‐tailed unpaired Student's t‐test.

Data information: Data are presented as mean ± SEM. P‐values are reported in Appendix Table S2.