Endothelial Notch signaling controls insulin transport in muscle

A

Uptake of FITC insulin and CAV1 expression in primary human umbilical venous ECs (HUVECs) upon Notch induction. Scale bar 20 μm.

B

Uptake of FITC insulin and CAV1 expression in (HUVECs) upon Notch blockage. Scale bar 20 μm.

C

Representative Western blot of AKT phosphorylation in gastrocnemius muscle 7 min after intravenous injection of PBS or 1.5 U/kg insulin in control (n = 3) and Rbpj ^i∆EC (n = 3) mice 5 weeks after recombination.

D

Densitometric analysis of Western blot in (C). n = 3, data represent mean ± SEM, unpaired t‐test.

E

Representative Western blot of AKT phosphorylation in liver 7 min after intravenous injection of PBS or 1.5 U/kg insulin in control (n = 3) and Rbpj ^i∆EC (n = 3) mice 5 weeks after recombination.

F

Densitometric analysis of Western blot in (E). n = 3, data represent mean ± SEM, unpaired t‐test.

Figure 3. Loss of Notch signaling improves endothelial insulin transport.