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. 2020 Mar 18;12(4):e09271. doi: 10.15252/emmm.201809271

Figure EV5. Analysis of caveolar genes in HUVECs and Rbpj iΔ EC mice on different diets.

Figure EV5

  • A
    Quantitative RT–PCR detection of CAVIN2 and CAVIN3 in primary human umbilical venous endothelial cells (HUVECs) upon Notch blockade (dnMAML) and induction (NICD). n = 5, data represent mean ± SEM, unpaired t‐test.
  • B
    Expression of HEY1, HEY2, and HES1 in HUVECs upon Notch manipulation. n = 4, data represent mean ± SEM, unpaired t‐test.
  • C
    Representative Western blot of CAV2 expression in HUVECs upon Notch manipulation.
  • D
    Densitometric analysis of Western blot in (C). Data represented as fold change over LacZ control. n = 5, data represent mean ± SEM, unpaired t‐test.
  • E
    Schematic illustration of feeding and recombination protocol.
  • F
    Weight curve of control or Rbpj iΔEC mice kept on control diet (CD, 10% fat, 70% carbohydrates) or high‐fat diet (HFD, 60% fat, 20% carbohydrates). n = 4 control CD, n = 5 Rbpj iΔEC CD, n = 6 control HFD, n = 4 Rbpj iΔEC HFD, data represent mean ± SEM.
  • G
    Blood glucose levels of control (n = 9) or Rbpj iΔEC (n = 9) mice kept on CD. Data represent mean ± SEM, unpaired t‐test.
  • H
    Blood glucose levels of control (n = 11) or Rbpj iΔEC (n = 10) mice kept on HFD. Data represent mean ± SEM, unpaired t‐test.