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Human antithrombin and murine antithrombin (0–5 μM) were added to wells coated with monovalent sdAbs (5 μg/ml). Bound antithrombin was probed using polyclonal anti‐antithrombin antibodies and detected via hydrolysis of 3,3′,5,5′‐tetramethylbenzidine. Plotted is the observed OD at 450 nm versus the antithrombin concentration.
