Skip to main content
. 2020 Mar 11;12(4):e11298. doi: 10.15252/emmm.201911298

Figure 4. Optimization of a liver‐specific vector expressing a secretable KB‐AT‐23 sdAb variant.

Figure 4

  1. Schematic representation of the KB‐AT‐23 expression cassettes used to test the secretion signal peptides SSP1, SSP2, SSP3, SSP4, and SSP5. hAAT: human alpha‐1 antitrypsin promoter; His: N‐terminal 6×‐Histidine tag; HA: C‐terminal tag derived from the human influenza hemagglutinin; BGHpA: bovine growth hormone polyadenylation signal.
  2. Western blot analysis of KB‐AT‐23 protein secretion in conditioned media 72 h post‐transfection of HuH7 cells. In the lower panel, the Western blot quantification via densitometry analysis is depicted. Data are presented as mean ± SD and were analyzed via 1‐way ANOVA with Dunnett's correction for multiple comparisons. ****P < 0.001.
  3. HuH7 cells were collected 72 h post‐transfection. Presented is the Western blot analysis of lysed cells for the presence of KB‐AT‐23. Cntr‐ refers to non‐transfected cells, while Cntr+ refers to purified recombinant KB‐AT‐23.

Source data are available online for this figure.