Skip to main content
. 2020 Mar 4;12(4):e11101. doi: 10.15252/emmm.201911101

Figure EV2. SCC tumour cells are dependent on USP28 and/or ΔNp63 to maintain a SCC identity.

Figure EV2

  • A
    Immunoblot of endogenous ΔNp63 and USP28 in A‐431 cells stably transduced with shRNA‐non‐targeting control (NTC) and two shRNA against ΔNp63. Actin served as loading control. n = 3.
  • B
    Cell growth of A‐431 cells stably transduced with shRNA‐non‐targeting control (NTC) and two shRNA against ΔNp63. Total cell number was measured in triplicate and assessed at indicated time points.
  • C
    Cell cycle profile analysis by propidium iodide staining of stable ΔNp63 knock‐down A‐431 cells by two independent shRNA sequences. n = 3.
  • D
    Immunoblot of endogenous ΔNp63 and USP28 in A‐431 cells stably transduced with shRNA‐non‐targeting control (NTC) and two shRNA against USP28. ACTIN served as loading control. n = 3.
  • E
    Cell growth of A‐431 cells stably transduced with shRNA‐non‐targeting control (NTC) and two shRNA against USP28. Total cell number was measured in triplicate and assessed at indicated time points.
  • F
    Cell cycle profile analysis by propidium iodide staining of stable USP28 knock‐down A‐431 cells by two independent shRNA sequences. n = 3.
  • G
    Gene set enrichment analyses of USP28#1‐silenced A‐431 cells compared to shRNA‐NTC using the gene list: “Genes Up‐regulated sh‐ΔNp63”. NES, normalized enrichment score; P < 0.0001.
  • H
    Gene set enrichment analyses of ΔNp63‐silenced A‐431 cells compared to shRNA‐NTC using the gene list: “Genes Up‐regulated sh‐USP28”. NES, normalized enrichment score; P < 0.0001.
  • I
    Relative expression of consensus markers for squamous cancer, as used in Fig 4, in a pan‐cancer panel (GEPIA software).
  • J
    Gene set enrichment analyses of USP28‐silenced A‐431 cells compared to shRNA‐NTC using the gene list: “Hallmark NOTCH Signaling”, “Hallmark MYC targets V1” and “PID AP1 Pathway”. NES, normalized enrichment score.
  • K
    Gene set enrichment analyses of ΔNp63‐silenced A‐431 cells compared to shRNA‐NTC using the gene list: “Hallmark NOTCH Signaling”, “Hallmark MYC targets V1” and “PID AP1 Pathway”. NES, normalized enrichment score.
Data information: All quantitative data are represented as mean ± SD; *P < 0.05, **P < 0.01. Two‐tailed t‐test. See also Appendix Table S3 (exact P‐values and statistical test used).