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. 2020 Mar 4;12(4):e11101. doi: 10.15252/emmm.201911101

Figure EV5. Pharmacologic inhibition of USP28 with AZ1 reduces tumour growth in an orthotopic model of lung SCC tumours.

Figure EV5

  • A
    Macroscopic and histological (representative H&E stainings from liver, pancreas and intestine) analysis of organs from animals treated with either control, solution, 125 or 375 mg/kg AZ1 (scale bars = 100 μm); n = 3.
  • B
    Schematic model for total tumour protein extraction from animals shown in (A).
  • C
    Representative ubiquitin‐suicide probe immunoblot of endogenous USP28 from tumour explants as shown in (B). Shown are low (l.e.) and high exposure (h.e.) images of the same USP28 blot. Values indicate relative % of active or inactive USP28. Representative immunoblot of endogenous ΔNp63 from control or AZ1‐treated animals. Values indicate relative expression of ΔNp63. ACTIN served as loading control; n = 3.

Source data are available online for this figure.