Skip to main content
. 2020 Mar 5;12(4):e10895. doi: 10.15252/emmm.201910895

Figure 1. Overexpression of FLT3/ITD induced axis duplication and ectopic expression of FST in zebrafish embryos.

Figure 1

  • A–D
    The morphology of uninjected, FLT3/WT mRNA, and FLT3/ITD mRNA‐injected (150 ng per embryo) embryos on day 2 post‐fertilization (dpf).
  • E–H
    Whole‐mount in situ hybridization (WISH) of notochord‐specific marker col9a2 in uninjected, FLT3/WT mRNA, and FLT3/ITD mRNA‐injected embryos on 2 dpf.
  • I, J
    FLT3 signaling was detected by Western blotting in 293FT cells transfected with FLT3/ITD mRNA (I) or in zebrafish embryos injected with FLT3/ITD mRNA (J).
  • K
    The effect of FLT3 inhibitor quizartinib (Qui) on the dorsalization and axis duplication phenotype induced by FLT3/ITD mRNA injection in zebrafish.
  • L–N
    Quantification of fst expression by RT–qPCR (L), Western blotting (M), and WISH (N) after FLT3/ITD overexpression in zebrafish embryos at 6 hpf.
Data information: ov: otic vesicles; cm: cephalic mesoderm; nc: notochord. Scale bar = 500 μm. In (K and L), the experiments were performed in triplicates and the data are presented as mean ± SEM. *P < 0.05 and **P < 0.01 (Student's t‐test). NS, not significant.Source data are available online for this figure.