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. 2020 Mar 1;13(3):357–370.

Figure 7.

Figure 7

Silencing ANXA7 affects miR-124-3p related protein in Hca-F cells. A. The expression vector ANXA7 and its unrelated sequence were transfected into Hca-F cells by Lipofectamine 2000. The green fluorescent protein was observed under fluorescence microscope after incubation at 37°C for 24-48 hours. B. The levels of ANXA7 gene in Hca-F group, N-control group, and shRNA-Anxa7 group were detected by qPCR. C, D. Western blot was used to detect the levels of ANXA7 protein in Hca-F group, N-control group, and shRNA-Anxa7 group. E. q-PCR was used to detect the levels of Bax, Bcl-2, MMP-9, and CXCL12 gene in the Hca-F group, N-control group, and shRNA-Anxa7 group. F, G. Western blot was used to detect the levels of Bax, Bcl-2, MMP-9, and CXCL12 protein in the Hca-F group, N-control group, and shRNA-Anxa7 group. The error bar represents the SD (n = 3). *P < 0.05, **P < 0.01, ns P > 0.05.