Immune cell expressions in urinary bladder by day 60 after CC induction. A-C. Illustrating microscopic finding (200 ×) of immunohistochemical (IHC) stain for identification of cellular expression of CD4 (gray color). D. Analytical result of CD4 expression, * vs. other groups with different symbols (†, ‡), P < 0.0001. E-G. Illustrating IHC microscopic finding (200 ×) for identification of cellular expression of CD8 (gray color). H. Analytical result of CD8 expression, * vs. other groups with different symbols (†, ‡), P < 0.0001. Scale bars in right lower corner represent 50 µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). Symbols (*, †, ‡) indicate significance at the 0.05 level. NC = normal control; CC = chronic cystitis; ECSW = extracorporeal shock wave.